Cross-reactions in specific Brachyspira spp. PCR assays caused by “Brachyspira hampsonii” isolates
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چکیده
منابع مشابه
First Identification of “Brachyspira hampsonii” in Wild European Waterfowl
Anseriformes deserve special attention in the epidemiology of Brachyspira spp. because diverse Anseriformes species have been described to act as highly efficient carriers of several Brachyspira spp. that can also infect livestock. The aim of this study was to investigate the prevalence and diversity of Brachyspira spp. in waterfowl that winter in Spain. Brachyspira spp. were isolated from 51 o...
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BACKGROUND Brachyspira associated diarrhea is a re-emerging concern for Canadian swine producers. To identify critical control points for reducing the impact of Brachyspira on production, improved diagnostic tools and a better understanding of the on-farm epidemiology of these pathogens are required. A cross-sectional study was conducted for the detection of Brachyspira on a commercial, two-sit...
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A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicolihipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicolihipp- with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilit...
متن کاملPCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces.
Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B. pilosicoli respectively were 2x10(2) and 7x10(3) cells p...
متن کاملIntestinal colonization of different Brachyspira spp. in laying hens
BACKGROUND:Avian intestinal spirochetosis (AIS) is causedby spiral-shaped Gram-negative Brachyspira spp. in poultry. It isknown as a cause of diarrhea, low egg production, and increasedoccurrence of dirty eggs in layer hens. OBJECTIVES: In this study,the presence of some Brachyspira spp. was investigated in layinghens. METHODS: A total of 100 cloacal swab samples wereindividually collected from...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2016
ISSN: 1040-6387,1943-4936
DOI: 10.1177/1040638716667528